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Education 2002 Ph.D. in BiochemistryUniversity of Rochester School of Medicine and Dentistry, Rochester, NY Advisor: Dr. Robert A. Bambara Dissertation: Molecular Mechanisms of Mammalian DNA Base Excision Repair Elmer Stotz Graduate Fellowship in Biochemistry 2000 M.S. in Biochemistry University of Rochester School of Medicine and Dentistry, Rochester, NY 1994 B.A. in Biology, Concentration in Physiology and Anatomy Cornell University College of Arts and Sciences, Ithaca, NY Dean's List
Professional Experience 2002-Present Postdoctoral Research Associate, Department of Cancer Genetics, Roswell ParkCancer Institute, Buffalo, NY. Investigation of siRNA directed inhibition of telomerase activity in breast cancer cell lines as a potential anti-tumor therapy 1997-2002 Graduate Research Assistant , Department of Biochemistry and Biophysics,University of Rochester School of Medicine and Dentistry, Rochester, NY. Thesis work involves the biochemical functional analysis of the mammalian long-patch base excision repair mechanism 1995-1997 Technical Associate II , University of Rochester Department of Biology, Rochester,NY.1994-1995 Research Associate , Syracuse University Department of Biology, Syracuse, NY.Analyzed the role of histone acetylation in activation of gene-expression Mapped acetylation sites in histone proteins in vitro and in vivoDeveloped site-specific antibodies against post-translationally modified (phosphorylated, acetylated) histones Supervised Protein Core Facility Trained new graduate students and technical staff 1994,1993 Summer Research Internship , Bristol Myers-Squibb Corporation, Syracuse, NYBiochemistry and Drug Development Division. Optimized scale-up conditions for Three Enzyme Process utilized in manufacturing Cephalosporin antibiotics Purified novel phosphatase for use in production of Etoposide anti-cancer agent 1993-1994 Undergraduate Research , Cornell University, Department of Psychology, Ithaca, NY.Laboratory of Dr. Elliot Blass Analyzed effects of maternal cocaine addiction upon pain tolerance in neo-natal rat pups
Publications Ranalli, T.A., Tom, S., and Bambara, R.A. (2002) APE1 Coordinates FEN1 and DNA Ligase IJ. Biol. Chem. 277(44), 41715-41724. Ranalli, T.A. , DeMott, M.S., and Bambara, R.A. (2002) Mechanism underlying replicationJ. Biol. Chem. 277(3), 1719-1727. Tom, S., Ranalli, T.A., Podust, V.N., and Bambara, R.A. (2001) Regulatory Roles of p21 andJ. Biol. Chem. 276(52), 48781-48789. Bornarth, C. J.* , Ranalli, T.A.*, Henricksen, L. A., Wahl, A. F. and Bambara, R. A. (1999)Biochemistry 40, 13347-13354. *co-first authorship Howe, L., Ranalli, T. A., Allis, C. D., and Ausio, J. (1998) Transcriptionally active xenopusJ. Biol. Chem. 273(33), 20693-20696. (communication) Hendzel, M. J., Wei, Y., Mancini, M. A., Van Hooser, A., Ranalli, T., Brinkley, B. R., Bazett-Chromosoma 106(6), 348-360. Kuo, M. H., Brownell, J. E., Sobel, R. E., Ranalli, T. A., Cook, R. G., Edmondson, D. G., Roth,Nature 383(6597), 269-272. Brownell, J. E., Zhou, J., Ranalli, T., Kobayashi, R., Edmondson, D. G., Roth, S. Y., and Allis,Cell 84(6), 843-851. Technical Skills High Pressure/Performance Liquid Chromatography (HPLC), Fast Performance Liquid Chromatography (FPLC), Cell Culture, Protein expression and purification (bacterial, yeast, and mammalian), Immunocytochemistry, Antibody Development/Analysis, Western Analysis, Northern Analysis, Rnase Protection Assays, PCR, Various biochemical assays (including acetyltransferase, kinase, and ChIP), SDS-PAGE, EMSA, TAU-gel electrophoresis, DNA sequencing, siRNA, FACS analysis
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